Two new root endophyte and nematode cyst parasite species of the widely distributed genus Laburnicola

Abstract Fungal root endophytes, including the common form group of dark septate endophytes (DSEs), represent different taxonomic groups with potentially diverse life strategies. During surveys DSE communities and nematode cysts colonizing fungi, isolates representing Laburnicola ( Didymosphaeriaceae , Pleosporales ) lineages were discovered. Here we carried out a comprehensive study phylogenetic relationships taxonomy fungi collected from plant roots in Hungary, Mongolia, Kazakhstan eggs cereal cyst Heterodera filipjevi Turkey. In addition to morphology culture characteristics strains, four loci (internal transcribed spacer, partial large small subunit regions nuclear ribosomal DNA translation elongation factor 1-alpha) used infer molecular strains within . The found two distinct lineages, which are described here as novel species, nematophila L. radiciphila interaction plants nematodes was examined using vitro bioassays, revealed endophytic interactions parasitic eggs. Analyses similar ITS sequences public databases that members genus widely distributed characteristic microbiome, they reported parasites for first time.